Aquatic Organisms

ECT offers a wide range of aquatic ecotoxicological standard tests under GLP according to OECD and ISO guidelines. This includes tests with aquatic micro-organisms, algae, higher plants, crustaceans, insects and fish. Our standard species in fish tests are zebrafish (Danio rerio), fathead minnow (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss). We can adapt test designs to the specific requirements of a test substance. In addition, we further develop and conduct tests with non-standard test species and multi-species test systems, and we are familiar with species sensitivity distributions.

Aquatic Micro-OrganismsGuideline
Activated Sludge, Respiration Inhibition TestOECD 209 (2010)
Determination of the Inhibitory Effect of Water Samples on Light Emission of Vibrio fischeri (Luminescent Bacteria Test)ISO 11348-2 (2007)


Aquatic PlantsGuideline
Freshwater Algae and Cyanobacteria, Growth Inhibition TestOECD 201 (2011)
Freshwater Algal Growth Inhibition Test with Unicellular Green AlgaeISO 8692 (2012)
Determining the Tolerance of Green Algae to the Toxicity of Waste Water (Scenedesmus Chlorophyll Fluorescence Test) by way of Dilution SeriesDIN 38412-33 (1991)
Lemna sp. Growth Inhibition TestOECD 221 (2006)
Determination of the Toxic Effect of Water Constituents and Waste Water on Duckweed (Lemna minor) – Duckweed Growth Inhibition TestISO 20079 (2005)
Water-Sediment Myriophyllum spicatum Toxicity TestOECD 239 (2014)
Sediment-Free Myriophyllum spicatum Toxicity TestOECD 238 (2014)
Water-Sediment Glyceria maxima Toxicity TestRing-test protocol (2022)


Aquatic CrustaceansGuideline
Acute Immobilisation Test (Daphnia magna); non-standard test species on request (e.g. Ceriodaphnia cf. dubia, Daphnia pulex, Daphnia hyalina/galeata) OECD 202 (2004)
Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea) – Acute Toxicity TestISO 6341 (2012)
Determining the Tolerance of Daphnia to the Toxicity of Waste Water by way of a Dilution SeriesDIN 38412-30 (1989)
Daphnia magna, Reproduction TestOECD 211 (2012)
Determination of Long Term Toxicity of Substances to Daphnia magna Straus (Cladocera, Crustacea)ISO 10706 (2000)
Ceriodaphnia cf. dubia Reproduction Testbased on ISO 20665:2008(E), OECD 211 (2012), EPA-821-R-02-013


Aquatic InsectsGuideline
Chironomus sp., Acute Immobilisation TestOECD 235 (2011)


FishGuideline
Fish Embryo, Acute ToxicityOECD 236 (2013)
Determination of the Acute Toxicity of Waste Water to Zebrafish Eggs (Danio rerio)ISO 15088 (2007)
Fish, Short Term Toxicity Test on Embryo and Sac-Fry StagesOECD 212 (1998)
Fish, Acute Toxicity TestOECD 203 (2019)
Fish, Juvenile Growth TestOECD 215 (2000)
Fish, Early-life Stage Toxicity TestOECD 210 (2013)
Fish, Partial Life-Cycle TestOECD DRP 95 (2008)
Fish, Complete Life-Cycle TestOECD DRP 95 (2008)


Fish (Endocrine Activity)Guideline
Fish, Short-Term Reproduction AssayOECD 229 (2012)
21-day Fish Assay: A Short-Term Screening for Oestrogenic and Androgenic Activity, and Aromatase InhibitionOECD 230 (2009)
Fish, Sexual Development TestOECD 234 (2011)
Fish, Partial and Complete Life-Cycle Test with non-standard endpoints on requestOECD DRP 95 (2008)

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