Terrestrial Organisms

ECT offers a broad range of terrestrial tests that includes all standard species usually required for risk assessment purposes in soil and dung. All tests can be conducted in compliance with GLP and according to OECD, EPA and ISO guidelines. If required, additional custom-designed studies can be performed, e.g. with modified exposure scenarios, and alternative test species can be used, e.g. to establish species sensitive distributions.

Soil Microorganisms: Nitrogen Transformation TestOECD 216 (2000)
Soil Microorganisms: Carbon Transformation TestOECD 217 (2000)
Contact Test for Solid Samples Using the Dehydrogenase Activity of Arthrobacter globiformisISO 18187

Terrestrial PlantsGuideline
Seedling Emergence and Seedling Growth TestOECD 208 (2006)
Vegetative Vigour TestOECD 227 (2006)
Determination of the Effects of Pollutants on Soil Flora. Part 1: Method for the Measurement of the Inhibition of Root GrowthISO 11269-1
Determination of the Effects of Pollutants on Soil Flora. Part 2: Effects of Contaminated Soil on the Emergence and Early Growth of Higher PlantsISO 11269-2
Chronic Toxicity in Higher PlantsISO 22030 (2005)

Soil InvertebratesGuideline
Earthworm Acute Toxicity TestOECD 207 (1984)
Effects of Pollutants on Earthworms – Part 1: Determination of Acute Toxicity to Eisenia fetida / Eisenia andreiISO 11268-1 (2012)
Tests for Toxicity of Contaminated Soil to Earthworms (Eisenia andrei, Eisenia fetida or Lumbricus terrestris)EPS1/RM/43, Environment Canada (2007)
Earthworm Reproduction Test (Eisenia fetida / Eisenia andrei)OECD 222 (2016)
Effects of Pollutants on Earthworms – Part 2: Determination of Effects on Reproduction of Eisenia fetida / Eisenia andreiISO 11268-2 (2012)
Enchytraeid Reproduction Test (several species)OECD 220 (2016)
Effects of Contaminants on Enchytraeidae (Enchytraeus sp.): Determination of Effects on ReproductionISO 16387 (2014)
Collembolan Reproduction Test in SoilOECD 232 (2016)
Inhibition of Reproduction of Collembola (Folsomia candida) by Soil ContaminantsISO 11267 (2014)
Predatory Mite (Hypoaspis (Geolaelaps) aculeifer) Reproduction Test in SoilOECD 226 (2016)
Avoidance test for determining the quality of soils and effects of chemicals on behaviour – Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei)ISO 17512-1 (2008)
Avoidance test for determining the quality of soils and effects of chemicals on behaviour – Part 2: Test with collembolans (Folsomia candida)ISO 17512-2 (2011)

Higher-tier Terrestrial StudiesGuideline
Terrestrial Model Ecosystems (TME), Semi-Field Test for Studying Effects and Fate of Chemicals in SoilAccording to PERAS
(Schaeffer et al. 2009)
Breakdown of Organic Matter in Litter Bags in the FieldOECD GD 56 (2006)
Effects of Pollutants on Earthworms – Part 3:
Determination of Effects in Field Situations
ISO 11268-3 (2014),
Technical recommendations according to Kula et al. (2006)
Field studies with soil invertebrates (enchytraeids, mites, collembolans and nematodes)ISO 23611-1 (2018),
ISO 23611-2 (2006),
ISO 23611-3 (2007),
ISO 23611-4 (2007),
ISO 23611-6 (2012)
Effects of soil contaminants on feeding activity of soil-dwelling organisms in the field – bait lamina testISO 18311 (2016)

Characterisation of Soil: Microbial CharacterisationGuideline
Determination of Soil Microbial Biomass – Part 1:
Substrate-Induced Respiration Method
ISO 14240-1 (1997)
Determination of Soil Microbial Biomass – Part 2:
Fumigation-Extraction Method
ISO 14240-2 (1997)

Characterisation of Soil: Physical / Chemical Characterisation 
• pH
• Texture
• Total organic carbon content
• Maximum water holding capacity
• Cation exchange capacity
• Total nitrogen content
• Carbonate content


ECT has expertise in the taxonomy of earthworms (Lumbricidae), potworms (Enchytraeidae), and nematodes. In several projects we have studied ecotoxicological effects on these groups of terrestrial invertebrates in combination with their ecology, distribution, and diversity in the field (monitoring studies). These projects are conducted throughout Europe as well as North and South America.

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