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Ring test with dung beetles

European legislation requires testing of parasiticides using, among others, dung beetles (OECD GD 122). Currently, Aphodius constans, a dweller species, has been approved as a test species for residues in dung. However, results of tests using A. constans may not be relevant for tunneler species.

Activated sludge test according to updated OECD guideline 209

For the activated sludge respiration inhibition test, the updated OECD test guideline 209 (22 July 2010) requires more replicates per treatment than the former guideline. To accomplish this new requirement and process the test within a reasonable timeframe, ECT recently established a multi-channel oxygen meter that allows simultaneous measurement and processing of respiration rates in […]

Validation of a sediment toxicity test with the endobenthic aquatic oligochaete Lumbriculus variegatus by an international ring test

The aim of the project was the validation of a sediment toxicity test with the benthic oligochaete Lumbriculus variegatus by an international ring test. The test method was developed taking into account existing methods. A test protocol was prepared and used to run the test. A total of 14 institutions (governmental agencies, universities, contract laboratories […]

Determination of developmental toxicity of a test chemical to Dipterian dung flies (Scathophaga stercoraria (Scathophagidae), Musca autumnalis (Muscidae))

The aim of the project was to standardise a test guideline with two dung fly species to be used for the environmental risk assessment of veterinary pharmaceuticals, in particular endo- and ectoparasiticides excreted in dung. The test is designed to estimate the developmental toxicity of a test substance to the dung dwelling life stages of […]

Supply of dung beetles for the performance of a ring test as a precondition for the implementation of an OECD Guidance Document

The objective of this project sponsored by the German Federal Environment Agency was the improvement of existing laboratory tests with dung beetles and dung flies. It was aimed at identifiying a second dung beetle species for standardised laboratory tests that should belong to the ecological group of ‘tunnelers’.  However, it was not possible to establish […]

Development of a standardised laboratory test with dung beetles for the assessment of the ecotoxicity of veterinary pharmaceuticals

According to European law, the environmental risk assessment of veterinary pharmaceuticals for dung beetles is required for parasiticides for the treatment of pasture animals. Starting with recommendations from the SETAC advisory group DOTTS (Dung Organism Toxicity Test Standardisation), a test system using the widespread temperate dung beetle species Aphodius constans was developed. Together with the […]